auramine oo
Basic Yellow 2
CAS: 2465-27-2
Molecular Formula: C17H22ClN3
auramine oo - Names and Identifiers
auramine oo - Physico-chemical Properties
| Molecular Formula | C17H22ClN3 |
| Molar Mass | 303.83 |
| Melting Point | >250°C (dec.)(lit.) |
| Boling Point | 406.2°C at 760 mmHg |
| Flash Point | 199.4°C |
| Water Solubility | Soluble in water or ethanol |
| Solubility | 10g/l |
| Vapor Presure | 8.31E-07mmHg at 25°C |
| Appearance | Yellow powder |
| Color | Yellow to ochre-yellow |
| Maximum wavelength(λmax) | ['370 nm, 432 nm'] |
| BRN | 4030061 |
| pKa | 9.8, 10.7(at 25℃) |
| PH | 6-7 (10g/l, H2O, 20℃) |
| Storage Condition | Inert atmosphere,Room Temperature |
| Stability | Stable. Incompatible with strong oxidizing agents. |
| MDL | MFCD00012484 |
| Physical and Chemical Properties | Melting Point: 267 water-soluble:<0.1g/100 mL at 18 C |
| Use | Gram O is used for the staining of the acid-fast organism coccidia. Gumamine O, together with carbol, produces a bright yellow fluorescent dye of Mycobacterium tuberculosis. It effectively detects positive cases of tuberculosis. Gram O binds to mycolic acid in the cell wall of bacteria. The Churukian modified Truant "s fluorescence method was used to determine the fluorescence microscope certification of acid-fast bacilli on paraffin sections. |
auramine oo - Risk and Safety
| Risk Codes | R22 - Harmful if swallowed R24 - Toxic in contact with skin R40 - Limited evidence of a carcinogenic effect R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. |
| Safety Description | S36/37 - Wear suitable protective clothing and gloves. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) S36 - Wear suitable protective clothing. S22 - Do not breathe dust. |
| UN IDs | UN 2811 6.1/PG 3 |
| WGK Germany | 3 |
| RTECS | BY3675000 |
| TSCA | Yes |
| HS Code | 3204 13 00 |
| Hazard Class | 6.1 |
| Packing Group | III |
| Toxicity | LD50 orally in Rabbit: 1000 mg/kg |
auramine oo - Introduction
Soluble in cold water, soluble in hot water is bright yellow, soluble in ethanol is yellow, its aqueous solution will be decomposed into tetramethylbenzophenone when the temperature exceeds 70 ℃, its aqueous solution is colorless when concentrated sulfuric acid is added, and it is light yellow after dilution. Solubility in water: 10g/l (20°C). Soluble in DMF and acetone. & & wavelength: λmax 370 nm, λmax 434 nm (2nd)
Last Update：2022-10-16 17:15:15
auramine oo - Reference Information
| color index | 41000 |
| pH indicator color change ph range | 6.5 |
| biological field application | Detecting bacteria; treatment of cancers,cardiovascular disease,diabetesassociated pain,mechanical allodynia,metabolic syndrome,protozoan infections in fish; targeted drug delivery; As fungal inhibitors |
| NIST chemical information | Information provided by: webbook.nist.gov (external link) |
| EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
| use | alkaline yellow o can be used for dyeing silk, cotton, acrylic fiber, wool, etc., and also for direct printing. when using, the dissolution temperature should not exceed 60 ℃. Because its sun fastness is too poor, it has been rarely used in textiles. Can be used for coloring leather, paper, paint, etc. It is used for acetic acid fiber and mordant cotton, but it has low fastness and bright color. It can be used to make green or red. It can also be used for dyeing leather, paper, hemp and viscose. Alkaline can be used for coloring oil, fat, paint, etc. It can also prepare lake for ink. It is mainly used for fluorescent staining of acid-resistant bacteria such as Mycobacterium tuberculosis. After the acid-resistant bacteria are stained with fluorescent dye Auramine O (gold amine O) and examined with a fluorescence microscope containing an ultraviolet light source, they will emit a shiny orange color. This method can be examined by lower times, so it can find acid-resistant bacteria more quickly. |
| production method | condensation of N,N-dimethylaniline and formaldehyde, after distillation and crystallization refining, ammoniation with sulfur, urea and ammonium chloride, then filtration and drying to obtain the finished product. Raw material consumption (kg/t)N,N-dimethylaniline (98%) 110 formaldehyde (37%) 460 urea 700 sulfur (99%) 350 ammonium chloride 630 p-aminobenzenesulfonic acid (100%) 8 refined salt 7500 method 1: melting method using N,N-dimethylaniline as the main raw material, firstly condensing with formaldehyde to obtain diaryl methane, which is refined by distillation and crystallization, ammoniated with urea, sulfur and ammonium chloride, filtered and dried to obtain the finished product.. The ammonification reaction is actually a three-step reaction of vulcanization, ammoniation and salting, that is, 4,4 '-dimethylaminodiphenylmethane, sulfur, urea and ammonium chloride are added to the ammonification kettle in proportion, the temperature is raised to (200±5)℃, the reaction is carried out for 4 hours, and the material is released. Method 2: Solvent method The newly developed solvent method uses ethylene glycol as solvent to reduce the reaction temperature and greatly improve the yield. The reaction process is as follows:. 300g of ethylene glycol and 58g of sulfur were put into the reactor, ammonia gas was introduced at (140±5)℃, 80g of ammonium chloride was added after 4h of reaction, and the ammonia gas was continued for 16h, with a total ammonia gas flux of about 102g. The reaction is finished, cooled, crystallized, filtered and dried to obtain about 155g of product. |
| category | toxic substances |
| toxicity classification | highly toxic |
| acute toxicity | oral administration-mouse LD50: 480 mg/kg; Abdominal cavity-rat LD50:135 mg/kg |
| flammability hazard characteristics | open flame is combustible; toxic nitrogen oxide gas is released by thermal decomposition |
| storage and transportation characteristics | warehouse ventilation and low temperature drying; separate from oxidant and food raw materials |
| fire extinguishing agent | carbon dioxide, sand, foam, mist water |
| toxic substance data | information provided by: pubchem.ncbi.nlm.nih.gov (external link) |
Last Update：2024-04-09 21:11:58
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